@MaerklLab

Synthetic Biology, Microfluidics and Diagnostics. Institute of Bioengineering - School of Engineering - Swiss Federal Institute of Technology @EPFL_en

 EPFL, Lausanne
lbnc.epfl.ch
Joined July 2013
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Cells, who needs them? Fun to be in @nature.com’s technology feature with @MichaelCJewett , @thenoireauxlab, @HNiederho, Wilson Wong, @LucksLab, Evan Karousis, Yan Zhang, Emily Chen & @cynbio01 🧪 nature.com/articles/d41586-0…

Cells, who needs them? Biochemists turn to proteins in a tube

Nature - Scientists are using cell-free transcription and translation systems to synthesize proteins, design biology-based sensors and develop synthetic cells.

nature.com
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Jiami and Laura's paper is out now! In collaboration with the Reddy lab at ETHZ! cell.com/cell-systems/fullte…

High-throughput cell-free profiling of SARS-CoV-2 RBD variants enables rapid and quantitative in...

The team developed a high-throughput platform to rapidly measure how hundreds of viral mutation combinations affect antibody binding, generating 31,740 quantitative affinity data points. The result...

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We combined cell-free protein synthesis + microfluidics to rapidly map how SARS-CoV-2 mutations affect antibody binding — 518 RBD variants, ~31,700 affinity measurements, 3 binding-energy landscapes spanning WT to Omicron.
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Pao-Wan and co's paper is published now as well ! "A T7 RNAP regulatory toolbox for cell-free network engineering and biosensing applications" nature.com/articles/s41467-0…

A T7 RNAP regulatory toolbox for cell-free network engineering and biosensing applications

Nature Communications - T7 RNA polymerase is widely used in synthetic biology, but tools to precisely regulate it are limited. Here, authors engineer a modular T7 RNAP regulatory toolbox for...

nature.com
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Saeed's paper is now published: "PURE makes PURE: reconstitution of the PURE cell-free system from self-synthesized proteins" nature.com/articles/s41467-0…

PURE makes PURE: reconstitution of the PURE cell-free system from self-synthesized proteins

Nature Communications - Building a self-replicating biochemical system remains a challenge in synthetic biology. Here the authors demonstrate that the cell-free protein synthesis PURE system can be...

nature.com
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Maria's paper on characterizing trans-properties of transcription factors and how they affect gene expression is out now! biorxiv.org/content/10.64898…
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Saeed's latest paper is now out on bioRxiv: "PURE makes PURE: reconstitution of the PURE cell-free system from self-synthesized non-ribosomal proteins" biorxiv.org/content/10.64898…

PURE makes PURE: reconstitution of the PURE cell-free system from self-synthesized non-ribosomal...

Building a universal biochemical constructor, an autonomously self-replicating bio-chemical system, is a major challenge in synthetic biology. The PURE cell-free system is an ideal starting point for...

biorxiv.org
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Check out Amogh's latest review: "Microfluidics meets cell-free systems: from molecular engineering to synthetic cells" now published in Current Opinion in Biotechnology. sciencedirect.com/science/ar…
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Laura and Jiami's paper is out now on BioRxiv! Nice collaboration with the Reddy lab at ETHZ: "High-throughput cell-free profiling of SARS-CoV-2 RBD variants enables rapid and quantitative in vitro affinity landscape mapping." biorxiv.org/content/10.1101/…

High-throughput cell-free profiling of SARS-CoV-2 RBD variants enables rapid and quantitative in...

SARS-CoV-2 variants continue to threaten public health, necessitating the study of cumulative and epistatic effects of receptor-binding domain (RBD) mutations on antibody evasion. We present a...

biorxiv.org
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The Maerkl Lab is now on Bluesky (better late than never as they say...)! bsky.app/profile/maerkl-lab.…
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Fanjun and Amogh's paper is now published: "Continuous in situ synthesis of a complete set of tRNAs sustains steady-state translation in a recombinant cell-free system" nature.com/articles/s41467-0…

Continuous in situ synthesis of a complete set of tRNAs sustains steady-state translation in a...

Nature Communications - Building autopoietic biochemical systems requires in situ tRNA synthesis, which remains challenging. Here, authors achieve continuous production of all 21 tRNAs in a PURE...

nature.com
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Pao-Wan, Saeed and Matthis' paper is out now on BioRxiv: "A T7 RNAP regulatory toolbox for cell-free network engineering and biosensing applications" biorxiv.org/content/10.1101/…
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Fanjun and Amogh's paper is out now on BioRxiv: "Continuous in situ synthesis of a complete set of tRNAs sustains steady-state translation in a recombinant cell-free system." biorxiv.org/content/10.1101/…

Continuous in situ synthesis of a complete set of tRNAs sustains steady-state translation in a...

Construction of a self-regenerating biochemical system is critical for building a synthetic cell. An essential step in building a self-regenerative system is producing a complete set of tRNAs for...

biorxiv.org
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Laura and Laura's paper is out now in ACS Synthetic Biology. "Long-Term Protein Synthesis with PURE in a Mesoscale Dialysis System" pubs.acs.org/doi/full/10.102…

Long-Term Protein Synthesis with PURE in a Mesoscale Dialysis System

Abstract. Cell-free systems are powerful tools in synthetic biology with versatile and wide-ranging applications. However, a significant bottleneck for the

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Pao-Wan's review on cell-free regulatory components is out now! Regulatory Components for Bacterial Cell-Free Systems Engineering | ACS Synthetic Biology pubs.acs.org/doi/10.1021/acs…

Regulatory Components for Bacterial Cell-Free Systems Engineering

Abstract. Cell-free systems are advancing synthetic biology through fast prototyping and modularity. Complex regulatory networks can now be implemented in

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Laura (@SuNMIL_EPFL) and Laura's paper on running the PURE system for weeks using a simple mesoscale dialysis system is now on bioRxiv!! "Long-term protein synthesis with PURE in a mesoscale dialysis system" biorxiv.org/content/10.1101/…

Long-term protein synthesis with PURE in a mesoscale dialysis system

Cell-free systems are powerful tools in synthetic biology with versatile and wide-ranging applications. However, a significant bottleneck for these systems, particularly the PURE cell-free system, is...

biorxiv.org
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The Maerkl Lab is hiring one or two graduate students to work in the area of cell-free synthetic biology, specifically synthetic cell engineering! Deadline for our EDBB doctoral program is Nov 1st ... See below for more information: eth-gethired.ch/de/jobs/deta…
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Ragu's paper is now officially out in ACS SynBio: Towards Self-regeneration: Exploring the Limits of Protein Synthesis in the Protein Synthesis Using Recombinant Elements (PURE) Cell-free Transcription–Translation System | ACS Synthetic Biology pubs.acs.org/doi/10.1021/acs…

Towards Self-regeneration: Exploring the Limits of Protein Synthesis in the Protein Synthesis Using...

Abstract. Self-regeneration is a key function of living systems that needs to be recapitulated in vitro to create a living synthetic cell. A major limiting

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Laura's paper from the Stellacci lab (@frstella) is out now: Nature-Inspired Recycling of a Protein Mixture into a Green Fluorescent Protein-based hydrogel - now published in RSC Sustainability pubs.rsc.org/en/content/arti…

Nature-inspired recycling of a protein mixture into a green fluorescent protein-based hydrogel†

Protein-based materials are biocompatible and have a variety of remarkable properties; consequently, they are finding more and more applications. Nature re

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Amogh's comprehensive review of microfluidic approaches and applications in cell-free synthetic biology is out now! #microfluidics #cellfreesynbio #synbio #syntheticbiology frontiersin.org/articles/10.…
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