@SternbergLab

News from the Sternberg lab at Columbia Univ., HHMI. Posts are from lab members and not Samuel Sternberg unless signed SHS. Posts represent personal views only.

 New York, NY
sternberglab.org
Joined October 2017
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1/7 Last week the Sternberg Lab welcomed ~40 junior & senior high school AP Research students from the East Meadow school district (and their incredible teachers) into our lab at Columbia University.
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6/7 And they enjoyed this agarose gel meme, made especially for our young friends ;)
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7/7 As a lab we believe in sharing both our science and our research knowledge with others, and hope we’ve inspired a few minds! Looking forward to the next visit!
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1/5 Telomerase has long been linked to chromosome maintenance in eukaryotes. Now, we show that telomerase-like activity is also present in bacteria, shedding new light on its ancient origins. Excited to share that our work on DRT10 is now out in @Nature! nature.com/articles/s41586-0…

Antiviral immunity via telomerase-like reverse transcription in bacteria

Nature - Antiviral immunity via telomerase-like reverse transcription in bacteria

nature.com
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4/5 DRT10 biochemistry and structure (with @IsraelF96135088): xitter.cf/SternbergLab/status/20…
Sternberg Lab@SternbergLabJul 9
1/11 New preprint from the Sternberg lab in collaboration with the Fernández lab! We are excited to share our structure-function study of DRT10, a bacterial defense-associated reverse transcriptase that synthesizes long tandem-repeat DNA.🧵 More here: biorxiv.org/content/10.64898…

Mechanism of tandem-repeat DNA synthesis by an antiviral reverse transcriptase

Defense-associated reverse transcriptases (DRTs) employ DNA synthesis to protect bacteria against phage infection[1][1],[2][2]. We previously showed that DRT10, a tripartite system comprising an RT,...

biorxiv.org
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5/5 Huge congratulations to all the authors for their incredible work bringing this story together! It was a fantastic collaborative effort spanning time zones and continents.
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1/2 Here we report the activity of Arc, a 50-aa DNA-binding protein encoded within IS605-family transposons. biorxiv.org/content/10.64898…
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2/2 We used sequencing and fluorescence reporter assays to uncover Arc’s role as a transcriptional repressor. It binds the transposon promoter and regulates gene expression, suggesting a new mechanism for transposon control!
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1/13 — New pre-print from the Sternberg Lab in collaboration with Leifu Chang's Lab! TnpB nucleases have repeatedly given rise to TldRs, nuclease-dead RNA-guided transcription factors. Here we define the biological role of a TldR clade in its native host. biorxiv.org/content/10.64898…
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12/13 — TldR is a naturally evolved parallel to engineered CRISPRi. Repression of an OppA variant shifts the relative contribution of remaining paralogs, which could influence which peptides a cell imports. What induces TldR natively remains unknown. biorxiv.org/content/10.64898…

Structural and genetic dissection of RNA-guided gene repression by TnpB-derived transcription...

TnpB nucleases, the evolutionary progenitors of CRISPR-associated Cas12 enzymes, are transposon-encoded, RNA-guided endonucleases found throughout bacteria. Independent of the trajectory towards...

biorxiv.org
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13/13 — A joint effort between the Sternberg and Chang labs: Renjian Xiao, Americo Casas-Ciniglio, Henry Le, Tanner Wiegand, and Dan Xie, supervised by Leifu Chang and Sam Sternberg.
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