Developmental biologist @COSHeidelberg @WittbrodtLab chalk-talker, CRISPRer, Baseeditor & glycosylation & CDG modeler.
Heidelberg
Joined October 2013
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Want to base edit and no PAM in reach? Just create one! "Inception" is a one-shot event based on a canonical target site to create a de novo PAM and a 2nd editing upstream, relaxing steric dependencies. #CRISPR #baseedit with @kaisa_pakari, @WittbrodtLab
biorxiv.org/content/10.1101/…
Calling all #medaka PI researchers! Join us in Heidelberg, Germany July 22-24, 2025 for our collaborative meeting! Register now meetmedaka.com/registration/ & take advantage of early bird rates until April 15th! 🐟 #MedakaResearch #ModelOrganisms #ScienceConference #FishModels #fish
Thomas Thumberger retweeted
New high-throughput optomotor response (OMR) assay for medaka fish larvae reveals strain-specific visual function differences & training effects. Our open-source setup enables studies on visual acuity, color sensitivity & more #NeuroBiology #FishResearch 🐠journals.plos.org/plosone/ar…
Thomas Thumberger retweeted
That's it: #GfE2024 is over. Thanks everyone for the science, amazing talks, chairing, buzzing poster sessions, short & long discussions, organizers in the back & front - simply thanks for all your contributions! See you all latest at #GfE2026 for updates & new routes of #DevBio
and furthered by comparison with other regenerating and non-regenerating fish like the surface and cave phenotypes of #astyanax. impressive talk at #GfE2024!
Mathilda Mommersteeg (@tmommersteeg) - as fish is not fish, "Identifying the metabolic profile underlying successful fish heart regeneration" is possible through the different #zebrafish strains available. #GfE2024
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very nice talk from @Adelmann_L on #platynereis. She makes use of #scTranscriptomics over time during the course of regeneration and finds stem cell hotspots in different clusters 💪 #GfE2024
Leonie Adelmann (@Adelmann_L) is "Dissecting the re-establishment of stem cells for hormone-dependent posterior regeneration in a marine annelid" #platynereis #stemcells #DevBio #GfE2024
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If you desperately need to explain the problem of in vitro recombination in #PCR fooling many to believe #CRISPR/#HDR events went smooth, use the remainder space on your poster 😂 read up here for fish elifesciences.org/articles/3… & here for the mouse academic.oup.com/nar/article… #GfE2024
Thomas Thumberger retweeted
Announcing the fantastic keynote speakers for the 11th AQMHD Conference: Leonard Zon, Jochen Wittbrodt, and Duygu Ozpolat. Registration and more information can be found here: aqmhd.xiphophorus.txst.edu/.
Thomas Thumberger retweeted
The member councils of the GfE and DSDB just met to discuss future plans #GfE2024. Common topic: high fluctuation of members. Join societies to support us in supporting events and young scientists - it's your annual fee that allows us to. Become a member: vbio.de/gfe-entwicklungsbiol…
Great talk by Christof about one of the hallmarks of #DevBio. Brings back memories of the good time I spent on the organizer during my PhD dissecting its downstream left-right organizing activity. #xenopus #cilia #LROrganizer
Christof Niehrs (@dkfz, @imbmainz) honours the famous Spemann-Mangold Organizer 100th anniversary experiment in his keynote lecture at #GfE2024 - the organizer through time #gastrulation #DevBio #frogs & #xenopus
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#GfE2024 last talk of the Stem cell session. Jochen talking about our SNP validation screen on heart-beat Gene-by-Environment alleles - CRISPR/BaseEditing SNP-fipping validated by CRISPR & ACEofBASEs editing aceofbases.cos.uni-heidelber…
Jochen Wittbrodt (@WittbrodtLab) "genetics of individuality" population genetics based on our inner fish. How SNPs influence traits like the 🫀 heart beat in a Gene-by-Environment dependency (GxE) #medaka #GWAS
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Thomas Thumberger retweeted
Open PhD position in Heidelberg. Please RT!
Do you want to identify the molecular mechanisms regulating genomic and chromosomal mosaicism in the developing brain? We seek a motivated doctoral researcher @COSHeidelberg @UniHeidelberg. Apply by 15 April here euraxess.ec.europa.eu/jobs/8…
CRISPR-Fortschritte - Schnitt für Schnitt zu neuen Möglichkeiten: unser Review über sukzessive, kleine Fortschritte, die gezieltes Genomeditieren mittels CRISPR & BaseEditoren effizienter gemacht haben
link.springer.com/article/10… ✂️ mit @kaisa_pakari & @WittbrodtLab im @Spektrum_BIO
I guess this meeting is focusing on zebrafish fin mutants 😉
Mark your calendars: The next Mid-Atlantic Regional #Zebrafish meeting will be held May 26th at the NIH! See our website for more information: sites.google.com/view/marz20…
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In contrast to PAM-less editors, inception uses target-specific NGG-editors. Chaining 2 guides & 2 editors is predictive & efficient. Though simultaneously applied, mode of action is sequential. 2nd edit depends on the 1st - allowing editing 27-36nt upstream of the canonical PAM
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published @Dev_journal now: journals.biologists.com/dev/…
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ahhh, bench air! Dear #miniprep, how I missed you! You routine chance for self-optimization. You simple process that grants room to think while doing, that makes automated movements happen thanks to imprinted memory in my Medulla spinalis. Even after years of abstinence. Thank U!
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Thomas Thumberger retweeted
Verfahren der Genom-Editierung optimiert: Heidelberger Wissenschaftlern gelingt es, die Effizienz von CRISPR/Cas9 sowie verwandten Methoden zu steigern und bislang unerreichbare DNA-Sequenzen zu modifizieren uni-heidelberg.de/de/newsroo…
ALT Nach erfolgreicher oca2-Gen-Editierung spiegelt das Verhältnis von ursprünglich pigmentierten zu unpigmentierten Zellen im Auge von Embryonen des Japanischen Reiskärpflings Medaka die Effizienz der Cas9-Aktivität wider. Dies wurde genutzt, um das CRISPR/Cas9-System zu optimieren. Wenig effektiver oca2-Verlust mit gängigen Cas9-Varianten (oben) und stark gesteigerte Effizienz durch Nutzung der heiCas9 (unten). © Thomas Thumberger (COS)
Just published @Dev_journal, Kaisa (@kaisa_pakari) presents our "inception" approach to target previously inaccessible nucleotides with base-editing at the 1st Heidelberg #CRISPR mini symposium @MKolleg today - link to paper: journals.biologists.com/dev/…
Thomas Thumberger retweeted
Here it is: our #inception paper @Dev_journal „De novo PAM generation to reach initially inaccessible target sites for base editing“ by @kaisa_pakari & @_ThomThum_ We use 2 guides & 2 editors to edit distant nucleotides with NGG base-editors
journals.biologists.com/dev/… #medaka #DNA