Johannes Stein (@jomo_stein) profile picture

Johannes Stein

@jomo_stein

Group Leader at Max Planck Institute for Molecular Genetics Studying genome organization with quantitative super-resolution microscopy https://xitter.gq/t.co/WPdMgmnoMx

Joined December 2017
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Photo by Johannes Stein (@jomo_stein)Photo by Johannes Stein (@jomo_stein)Photo by Johannes Stein (@jomo_stein)Photo by Johannes Stein (@jomo_stein)Photo by Johannes Stein (@jomo_stein)Photo by Johannes Stein (@jomo_stein)Photo by Johannes Stein (@jomo_stein)Photo by Johannes Stein (@jomo_stein)Photo by Johannes Stein (@jomo_stein)Photo by Johannes Stein (@jomo_stein)
Happy to share the published version of our study on the benefits of sodium sulfite + trolox (SST) as oxygen scavenger for #DNAPAINT - now out in Small! Thanks to the reviewers for helpful comments and again congrats, Becky, on your PhD's first paper!! tinyurl.com/54m2pt9c
A Simple, Ultrastable, and Cost‐Effective Oxygen‐Scavenging System for Long‐Term DNA‐PAINT Imaging

A Simple, Ultrastable, and Cost‐Effective Oxygen‐Scavenging System for Long‐Term DNA‐PAINT Imaging

A simple, enzyme-free, and cost-effective oxygen scavenging system enhances DNA-PAINT super-resolution microscopy by increasing photostability, minimizing docking strand degradation, and enabling...

onlinelibrary.wiley.com
Johannes Stein (@jomo_stein) profile pictureJohannes Stein@jomo_stein21 Jul 2025
(1/n) A simple, ultrastable, and cost-effective oxygen-scavenging system for long-term DNA-PAINT imaging Please check out our new study on the benefits of sodium sulfite as oxygen scavenging system for DNA-PAINT. 👇🧵 #SuperResolution #DNAPAINT #SMLM #Microscopy
Image from Johannes Stein (@jomo_stein)
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(1/n) A simple, ultrastable, and cost-effective oxygen-scavenging system for long-term DNA-PAINT imaging Please check out our new study on the benefits of sodium sulfite as oxygen scavenging system for DNA-PAINT. 👇🧵 #SuperResolution #DNAPAINT #SMLM #Microscopy
Image from Johannes Stein (@jomo_stein)
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(9/n) Curious to try it? 📄 Read the full preprint: biorxiv.org/content/10.1101/… We would love to hear your feedback!
A simple, ultrastable, and cost-effective oxygen-scavenging system for long-term DNA-PAINT imaging

A simple, ultrastable, and cost-effective oxygen-scavenging system for long-term DNA-PAINT imaging

DNA-PAINT (Points Accumulation in Nanoscale Topography) is a super-resolution microscopy technique capable of nanoscale imaging through the transient binding of fluorescently labeled imager strands...

biorxiv.org
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(10/n) Huge thanks to Becky Perelman for carrying out this work as her rotation project in the @geochurch lab – and congratulations on your first PhD paper🥳 🎉!
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Really excited to share this Perspective from John Danial covering how technology is moving the field of fluorescence microscopy toward structural biology. nature.com/articles/s41592-0…
Super-resolution microscopy for structural biology

Super-resolution microscopy for structural biology

Nature Methods - This Perspective introduces a framework for defining, measuring and reporting resolution in super-resolution microscopy and details the current state of the art in using...

nature.com
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Johannes Stein retweeted
Tinnefeld Lab (@LabTinnefeld) profile picture
Tinnefeld Lab@LabTinnefeld8 Nov 2024
1/5 DNA and DNA/protein conformations at Ångström/millisecond resolution—right in a fluorescence microscope! Check out our new GETvNA paper in @naturemethods : nature.com/articles/s41592-0… Want to learn about the science behind GETvNA?
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Excited to share it now in its final form in @NatureNano: our approach to decouple sensing from signal output to build modular and tunable DNA origami sensors with high FRET contrast: rdcu.be/dZqUd 💡🧬🔬
Image from Viktorija Glembockyte (@glembockyte)
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Nike Walther (@nike_walther) profile picture
Nike Walther@nike_walther31 Oct 2024
I am excited to share the first publication from my postdoc @TjianDarzacq lab @berkeleyMCB: “Automated live-cell single-molecule tracking in enteroid monolayers reveals transcription factor dynamics probing lineage-determining function” @CellReports cell.com/cell-reports/fullte…
Automated live-cell single-molecule tracking in enteroid monolayers reveals transcription factor...

Automated live-cell single-molecule tracking in enteroid monolayers reveals transcription factor...

Walther et al. develop an automated live-cell single-molecule tracking and analysis pipeline allowing imaging of hundreds of cells, the capture of cellular features, and correlation of these features...

cell.com
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Want to know what’s near your RNA of interest? Oligonucleotide-mediated proximity-interactome MAPping (O-MAP) from the Shechner lab enables precise multiomic characterization of biomolecular interaction networks (DNA, RNA, and proteins) at target RNAs. nature.com/articles/s41592-0…
Multiomic characterization of RNA microenvironments by oligonucleotide-mediated proximity-interac...

Multiomic characterization of RNA microenvironments by oligonucleotide-mediated proximity-interac...

Nature Methods - Oligonucleotide-mediated proximity-interactome MAPping (O-MAP) enables precise multiomic characterization of biomolecular interaction networks at target RNAs. Distinct O-MAP...

nature.com
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1/9 Insanely PROUD to share our new collaborative project with @Dey_Gautam & @CentrioleLab. #PlanExM We think (almost certain) #Expansion #Microscopy (#UExM) is a turning point for #Protistology and #Cell Biology. 🔗 [tinyurl.com/PlanExM1]
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Sofi Quinodoz (@sofiquinodoz) profile picture
Sofi Quinodoz@sofiquinodoz3 Oct 2024
Excited to share a new preprint! (1)🔬The nucleolus is the most prominent nuclear condensate, with a fascinating multilayered liquid-like structure, and is the site of ribosome biogenesis. But how does this multiphase architecture form and function? biorxiv.org/content/10.1101/…
Image from Sofi Quinodoz (@sofiquinodoz)
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Johannes Stein retweeted
zack chiang (@z_chiang) profile picture
zack chiang
@z_chiang26 Sep 2024
7 years ago, I met a junior fellow named @JD_Buenrostro who blew me away with a vision of futuristic genomic technologies Today, we (@ajaylabade31, @carolinecomenho) are excited to share our first steps into that future: Expansion in situ genome sequencing 1/
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The @naturemethods paper from Jürgen Plitzko's research group has made it onto the front page! Congratulations 🥳 ❕nature.com/nmeth/volumes/21/… Also read the latest News & Views article: ❕News & Views: nature.com/articles/s41592-0… ❕Publication: nature.com/articles/s41592-0… #CryoEM #CryoET
Image from Max Planck Institute of Biochemistry (MPIB) (@MPI_Biochem)
Max Planck Institute of Biochemistry (MPIB) (@MPI_Biochem) profile pictureMax Planck Institute of Biochemistry (MPIB)@MPI_Biochem18 Dec 2023
To improve #CryoET sample prep, researchers from the Plitzko lab developed Serial Lift-Out, a #cryoFIB based method that makes it possible to produce several lamellae from one sample block ❕biochem.mpg.de/anatomy-of-ne… ❕@naturemethods nature.com/articles/s41592-0… @svenklumpe @CJOKaiser
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