@lingboyu
 Eindhoven, the Netherlands
Joined April 2008
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12Media
Low-kV but high-res
Abhay Kotecha@AbhayKot9 Sep 2024
Building on the work of Richard and Chris, here is our latest work showcasing the potential of the 100 keV #Tundra microscope with the Ceta F and Falcon C. We achieved high-resolution structures for a variety of protein complexes🧵.🔬#100keV #CryoEM. Movie by @lingboyu
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Join us and get a close 👀 at🍩's. ter.li/vbj3dn
Adrian Koh@AdrianKoh84 Jul 2023
Results from new detector for @thermofishersci Tundra #CryoEM . Do I see some donuts? Join us on 7/13 at ter.li/vbj3dn to hear more about how we did it. Movie credit @lingboyu
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#cryoEM #MicroED with electron counting. Protein structure at 0.8A resolution. Ab initio phasing for two proteins facilitated by electron counting on a falcon 4 direct electron detector. A section of the map is shown below biorxiv.org/content/10.1101/…
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If the time slot on Nov 18th didn't work for you, the recording is now available on Tundra. thermofisher.com/us/en/home/… I had a lot of fun creating this video clip, learning on the job😊
lingboyu@lingboyu16 Nov 2021
Join us to take a close look at Tundra and the beautiful ❤️ structures Tundra produces. The event is on Nov 18, register now: ter.li/y43xvf
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Jiri was in our Tundra lab end of August this year. In this clip, you can have a sneak preview of our Tundra.
CEITEC@CEITEC_Brno17 Nov 2021
🔬Jiri Novacek, the Head of @cemcof, discusses how #cryoEM is accelerating his #PlantResearch by understanding the structure and function of the #PlumPoxVirus.👇He had the opportunity to try out a new Tundra #TransmissionElectronMicroscope by @thermofisher youtu.be/dOeJuACtTas

Dr. Jiri Novacek on how cryo-EM is advancing his plant research

Dr. Jiri Novacek from CEITEC, Masaryk University, discusses how cry...

youtube.com
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Join us to take a close look at Tundra and the beautiful ❤️ structures Tundra produces. The event is on Nov 18, register now: ter.li/y43xvf
Abhay Kotecha@AbhayKot16 Nov 2021
Replying to @DimpleKaria3
@DimpleKaria3 and @lingboyu will show some very high resolution structures from #Tundra and several screening cases of challenging proteins #CryoEM at 100kV. Join on 18th Nov to see more.
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Totally agree. Shot #1 and #2 look like purely for the number. Without touching the carbon support, these would normally suffer greatly from charging and do not contribute to the high resolution, unless there is conductive support over the hole, such as carbon.
とりさん@biochem_fan24 Jun 2021
Replying to @biochem_fan
This is impressive but the most important thing is to establish the best strategy to get the highest resolution (not the highest number of particles or movies) within a given scope time.
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#CAFR, 2.6Å map of Apoferritin, 6hrs of data with Ceta-F from Tundra (100kV cryo-TEM)
Abhay Kotecha@AbhayKot14 Sep 2021
Another 1.2Å map of Apoferritin #CAFR, now from just 6hrs of data with new Falcon 4i detector. 2 exposures per/hole and data processed with #Relion4 alpha
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Join us tomorrow for @labroots webinar, @SteveBrohawn and I will share latest work on membrane proteins, sub-100kDa structures, #SBDD with #KriosG4 CFEG Selectris Falcon4. I will also show latest results from 200kV #Glacios Selectris Falcon4. Register at labroots.com/ms/webinar/cryo…

Cryo-Electron Microscopy: membrane proteins down to atoms

Date: April 29, 2021 Time: 9:00am PST Cryo-electron microscopy (cryo-EM) reached single-atom resolution for 3D structure determination of biological macromolecules. Cryo-EM has been determin

labroots.com
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Preprint👇 updated with SARS-CoV-2 3a ion channel, 62KDa membrane protein at 2.1Å! Another wonderful work by @SteveBrohawn group @kerndm and @chrismhoel pushing #CryoEM
David Kern@kerndm26 Jan 2021
SARS-CoV-2 3a preprint update, 2.1Å! map with @AbhayKot @thermofisher, processed by me and @chrismhoel. We now have new PL uptake assays and RuR as an inhibitor from @sonalimali6 (Bautista Lab) and new 3a mutants patched by Ben Sorum. Led by @SteveBrohawn biorxiv.org/content/10.1101/…
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The pixel size used is 0.73A, not ridiculously high, I think. 1) Ceta at 100kV is way better than Ceta at 300kV 2) This Ceta has 4x better SNR compared to a normal Ceta.
Christos Savva@ChristosGSavva18 Nov 2020
Replying to @JNB_lab
How is the CETA reaching such high resolution? Higher mag to push to better DQE region?
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It is really easy, as easy as Krios/Glacios, basically only to put grids into the cassette. 5 grid/hour?🤔We need to put you to the test😂
Rado Danev@RadoDanev18 Nov 2020
Seriously, I like the Tundra!🔬🙂👍 If the loading is indeed very easy and quick, put a plunger next to it and plunge-load-plunge-load-... until you get the perfect grid for the bigger microscopes.😍
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I am thrilled to be involved in this project and also very excited to work with @WimJHH as our beta collaborator
Abhay Kotecha@AbhayKot16 Nov 2020
We are launching an affordable new cryo-EM instrument. Guess the resolution of this structure 🤪by @dimplekaria3 and @lingboyu. Join us to learn more about this new microscope which will extend this cutting-edge technique to all researchers. Register here: ter.li/yaptff
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Join us: ter.li/yaptff
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Oops, how I forget @SimoneMattei19, our amazing beta collaborator.
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Replying to @MartinWalshDLS
@MartinWalshDLS here is a pumpkin version with some wizardry from @lingboyu. There is also a pumpkin spiced #FSC @SjorsScheres as well. Lets make #Glacios great. Join on 5th November for more colors @AdrianKoh8 @WimJHH thermofisher.com/nl/en/home/…
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Look at this beauty!
Abhay Kotecha@AbhayKot3 Jun 2020
A nice piece from @nature news on our and Holger Stark’s @biorxivpreprint ‘It opens up a whole new universe’: Revolutionary microscopy technique sees individual atoms for first time nature.com/articles/d41586-0…
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Next "Ask the Expert" event is taking place today at 3 pm BST and will focus on EPU software. Join to hear about the latest features and tricks on how to speed up the set up: thermofisher-events.webex.co…
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Another EER data set to play with
とりさん@biochem_fan27 May 2020
New entry EMPIAR-10424 "Atomic resolution structure of apoferritin" (1.22 Å) ebi.ac.uk/pdbe/emdb/empiar/e… 3370 Falcon4 movies in EER (538.1 GB) Please use "devel-eer" branch of RELION 3.1 to process: github.com/3dem/relion/tree/…
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