Mostly Bacillus | unpublished data | hypotheses | wild ideas | sand-outta-box | who wants 2 bet? | 🧠🔥🐳 | Zinc ♡ | Main: Jen @JukeBaRosh | No one important |
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Joined June 2021
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Acct for sharing Bacillus-related stuff, but guaranteed to go off topic, b/c that's just what I do
Hypotheses made to be broken
#uncharacterizedgenes
#metabolism
#bacillussubtilis
#jukebaroshhypotheses
"CsfB assembles into a symmetrical homodimer, with each monomer bound to a single Zn2+ ion via a treble-clef zinc finger fold. Directed mutagenesis indicates that dimer formation is critical for CsfB-mediated inhibition of both σG and σE"
sciencedirect.com/science/ar…
nature.com/articles/s4146… "Rather, by directly inhibiting ATP synthase, bedaquiline slows the respiratory supercomplex (Qcr:Cta; bcc:aa3) by increasing the proton-motive force, causing sub-second redirection of electron flux through the cytochrome bd oxidase (CydAB) to O2."
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Also seen if you inoculate Bs168 wt next to some Bs168 mutants:
phoR
wapA/wapI
tuaD
ptpZ
ptkA
pstBA
The obv connection here is phosphate - in low phosphate Bacillus synthesizes different surface molecules. Teichuronic acid instead teichoic acid.
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1/ Made observation of germinating Bacillus subtilis spores & wondering if anyone knows what's going on
The cells were sporulated expressing a GFP-tagged, soluble, cytoplasmic protein
In overlay, see phase bright spores, germinating cells w/spore coat, & empty, cracked coats
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4/ reminded me of Bdellovibrio or a placenta- like a little sack of stuff around the spore core. But surely this would be visible by electron microscopy, so where is the GFP signal coming from?
Doing a totally unrelated GFP protein now and seeing the same thing. It's weird.
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"Cellular ATP demand creates metabolically distinct subpopulations of mitochondria"
nature.com/articles/s41586-0…
On Wed morning, opened email to subject line : [person's name] eulogy
Totally forgot I asked students to write their own eulogies as a reflection on their values (scientific/personal). Thankfully all are alive & well
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BacillusBaRosh 🌪️ retweeted
Checkout @Bevika2 preprint! She used CRISPRi-seq to determine antibiotic-stress signatures, specifically of pneumococci treated with fluoroquinolone antibiotics. It's a really powerful approach revealing a surprising antibiotic combination therapy. 1/9 biorxiv.org/content/10.1101/…
Meet Chonkus - a CO2-sequestering cyanobacterium that sinks in H20
journals.asm.org/doi/10.1128…
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Why does this matter?
Biofuel plants sequester C02 (good) & are a renewable source of energy (good), but they also burn to CO2 (bad).
Current strategies to offset CO2 include dissolving in saline aquifers.
An even better option would be to feed it to another C02 sequesterer.
"New Investigator"
5 yr PhD
6 yr post-doc
13.5 yr faculty
Just called it failed ESI - would be less patronizing
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I even know they cannot be called true L-forms unless they were induced in the Newcastle region of UK...
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