Neuronal Cell Biology. CryoEM.

Hershey, PA
Joined November 2017
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Replying to @FlyGutLab
Beautiful images and annotations! Congrats! I need to think about flies more.
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Replying to @CellJxn
It's only days away at this point! I can't wait to spread this very basic knowledge to everyone. The real training will begin in the not too distant future and we will use your samples for this.
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Turning the dials on our new Hydra pFIB for the first time today. Can't wait to get some cells into it, soon! Been freezing things down like mad for the past couple of weeks.
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🚨Members of #teamtomo!🚨 We invite you to submit abstracts to our ❄️🔬Cryo-electron Tomography: Progress and Potential session at Microscopy & Microanalysis (M&M) 2025! abstractscorecard.com/cfp/su… @benjaminbarad
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Replying to @CellJxn
Agreed. It's time to mill!
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It's alive! Still a ways to go but both beams are on.
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Ok...now that's pretty cool. Nice work everyone!
Excited to share our new preprint "Dynamic nanoscale architecture of synaptic vesicle fusion in mouse hippocampal neurons" biorxiv.org/content/10.1101/… We combine optogenetic stimulation of neurons with in situ cryo-ET to characterize membrane dynamics during vesicle fusion ❄️🔬⏱️
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Replying to @VirusEMLab
Looking forward to it!
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This delivery marks the dawn of a new era for cryo-ET at the Penn State College of Medicine. What should we mill into first?
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Congrats on your retirement and for leading such an inspiring career for so many of us! Good luck with everything!
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This was an awesome workshop. I'm lucky to have been a part of it. Hope to get an invite next year!
Great #cryoET #cryoFIB #sub-tomo workshop ⁦⁦@UWBiochem⁩ #cryoEM centers! New #teamtomo trainees ready, set, go! Thanks speakers, trainers, staff, sponsors. ⁦@NIH_CommonFund⁩ #cryoEM ⁦@delmicbv⁩ ⁦@SubangstromLLC⁩ @LeicaMicro⁩ ⁦@thermosciEMSpec⁩
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Someone needs to scoop this guy up quick!
I'm excited to announce that I am on the job market for tenure-track Assistant Professor positions in cell biology, with a focus on the integrated roles of keratin filaments and organelle biology! Please feel free to reach out, share/RT🔁!
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Replying to @FlorentWaltz
Completely stunning. Beautiful work!
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Replying to @parameciumbrain
Jess...it was an honor to have you in the lab. I'm proud of all you've accomplished and look forward to many more to come.
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Congrats Andrew and all involved!
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Replying to @rita_strack
I miss Texas so much. Glad you made it for one of the narrow windows of pleasant weather.
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That's quite the arsenal. Congrats!
Bacteria can be pirates too... 🏴‍☠️ Let us introduce you to Captain Aureispira ⛵️ A bacterial pirate ship loaded with canons 💉 and grappling hooks 🪝🌴predating on other bacteria 🦠 Check the preprint: biorxiv.org/content/10.1101/… #predation #T6SS #T9SS #cryoEM #cryoET #teamtomo 🧵1/6
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The "final form" of this beautiful paper is out in @NatureComms now! Hugely honored to be part of this with @_manasi_iyer_ and @ZucheroLab Particularly proud of the 3D serial section EM of myelin. Paper: link.springer.com/article/10…
What controls the morphology of #myelin sheaths to enable them to fine-tune conduction velocity? TL;DR: Calcium (Ca2+) signaling; a.k.a. nature’s ultimate multitasker! Read our new pre-print to find out more, led by grad student @_manasi_iyer_! 🧵👇(1/n)
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Wish I could be there!
Come chat with me tomorrow about deep learning segmentation for large scale analysis of cryo et and volume em data!! 1:45pm, board B4. #cellbio2023
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