ChemE PhD student @GallowayLabMIT
Cambridge, MA
Joined August 2020
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Emma Peterman retweeted
Congrats, @emmapeterman_ on a terrific defense!
An whirlwind journey to characterize gene elements at high resolution and to build improved circuit designs and frameworks that accelerate the Design-Build-Test-Learn loop!
Cheers to a job well done! Excelsior!
@MITChemE
Emma Peterman retweeted
Enjoyed the tweetorial? Want to hear more? Come hear
@emmapeterman_ live at AIChE this coming Thursday. Details 👇
Thursday, November 6th, 2025
Session #654: Cellular, Protein, & RNA Systems Engineering
8:00 AM, Hynes Convention Center, 310
Emma Peterman retweeted
So you want to change transgene expression: just change your promoter, right? Changing the promoter increases RNA and thus protein levels. What more could be happening?
[1/n] Well, promoters don’t just set RNA levels; they uniquely transform how RNAs are transmitted into protein levels. 🧵
Emma Peterman retweeted
Today at @CellSystemsCP we report in back-to-back papers (!) led by @nbwang22 on how cell state potentiates the influence of individual transcription factors to drive cell-fate programming
&
how we used these insights to scale up production of motor neurons from primary fibroblasts, allowing us to deliver these directly converted neurons into the brains of mice.
Updated tweetorial coming soon (links below!)
Emma Peterman retweeted
Your promoter is doing both more and less than you might expect.
@MITChemE
High-resolution profiling reveals coupled transcriptional and translational regulation of transgenes biorxiv.org/cgi/content/shor… #biorxiv_synbio
Emma Peterman retweeted
Stronger promoters not only transcribe more RNA but also show higher effective translation rates.
Emma Peterman retweeted
#FeaturedProtocol this week is for STRAIGHT-IN: a platform for rapidly generating panels of #geneticallymodified #humanpluripotentstemcell lines bit.ly/3yJXlVP @A_BlanchAsensio
Read about ComMAND below! Such a privilege to contribute to this excellent work lead by @kaseyslove
Loss of gene expression can compromise cellular fitness. In the case of such haploinsufficiency, replacement of the gene supplementation could rescue cell health. But what if you need a very precise dosage? Try ComMAND! 🧵 (1/n)
Link: doi.org/10.1101/2024.06.25.6…
Learned so much putting this review together with @DeonPloessl and @GallowayLabMIT 🧬 Stay tuned to hear about how we are approaching these challenges in the lab!
Emma Peterman retweeted
Levels of gene expression matter! So how can you control a transgene precisely? Is there a tool that can ensure uniform control & the ability to adjust the setpoint of expression from a single promoter? Introducing DIAL! 🧵 (1/n)
Link: doi.org/10.1101/2024.06.19.5…
Emma Peterman retweeted
Or stop by poster #28 to hear from Emma Peterman
@emmapeterman_
on her work on "Simultaneous measurement of protein and mRNA levels in single cells for genetic element characterization" in mammalian cells
#SEED2024 #synbio
@MITChemE
Emma Peterman retweeted
on unimodal setpoints and how to get them from synthetic promoters...
tweetorial coming soon...
or see you next Thursday #SEED2024 in Atlanta...
Stay tuned for more control systems coming soon...
Programmable promoter editing for precise control of transgene expression
ift.tt/RchaKp6
#biorxiv_synbio
Emma Peterman retweeted
Excited to say our lab's about to do some "understanding and engineering" with the support of the NSF CAREER Award! Really grateful for fantastic work by my students and support from my department @MITChemE Delighted for the research and outreach this will support. Excelsior!
Emma Peterman retweeted
1st preprint is finally out! When I started, as a 🤖 synbio person, there were so many Q's I had on how to build reprogramming vectors 🔧🧬. There's a lot more to do but we show how you encode reprogramming cocktail really matters in TF-specific ways! See tweetorial for more
How do individual transcription factors (TF) influence reprogramming? Isn’t it just the cells with the highest levels of TF expression that reprogram? So just blast them & it should work, right? 🤨Buckle up for our lab’s preprint & tour de force led by Nat Wang @nbwang22 ! (1/n)
Emma Peterman retweeted
How do individual transcription factors (TF) influence reprogramming? Isn’t it just the cells with the highest levels of TF expression that reprogram? So just blast them & it should work, right? 🤨Buckle up for our lab’s preprint & tour de force led by Nat Wang @nbwang22 ! (1/n)
Proliferation history and transcription factor levels drive direct conversion biorxiv.org/cgi/content/shor… #biorxiv_cellbio
Emma Peterman retweeted
If you’re at #ISSCR2023 , come see my poster #285 on Wed, 6pm!
Excited to share my work on how we’ve systematically optimized direct iMN reprogramming to explore and improve controllability of reprogramming in both 🐭 mouse and 👤 human!
Wed Jun 14th 6pm: Come to poster #285 and hear Nat Wang tell you about his work on the systems biology-guided design and molecular optimization of circuits for cell fate reprogramming. Nat also won an ISSCR Merit Award for his abstract! (1/n)
Emma Peterman retweeted
How does transcription of proximal genes influence the expression of their neighbors? How do we engineer compact circuit designs for specific profiles of expression that enable robust cellular engineering? Today @CellReports we provide some answers (1 /n) tinyurl.com/24vtfbyw
Excited to share my new website! Here's what I've been up to in my first year of grad school and some advice for incoming students. More updates to come! emmapeterman.netlify.app/