@GallowayLabMIT

Associate Prof @MITChemE ; mom^4 + wife; enjoys building cell-fate circuits, exploring dna topology, reprogramming the living world, and soccer; soli gloria deo

gallowaylab.mit.edu/
Joined June 2019
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Could the folding of synthetic gene circuits in 3D shape how genes are expressed? Today @ScienceMagazine we report on the role of gene syntax in shaping feedback between transcriptional activity and genome folding for advanced circuit design🧵 (1/n)
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Katie Galloway retweeted
Howard Salis@hsalis22h
Massively parallel assays using oligopools, barcoding, & deep sequencing follows the same story. Replaces 100s of microtiter plates (one assay per well) with 10000s of experiments run in one pooled assay. Work your 🧠, not just more 🤖.
Zach Rosenthal
@rosenthalzach1Oct 21
Maybe controversial: the conversation about scientific bottlenecks is overfocused on automation. New measurement technologies deserve more attention. You could automate an assay that needs an entire facility – or you could REINVENT the assay so that it fits into just a few tubes.
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Arjun Raj
@arjunrajlabOct 21
All cell types are wrong, but some are useful.
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Last year, we spent a lot of time thinking about to engineer some of the most complex cellular systems: lung, heart, blood, and sleep systems! In case you missed it, you should check out some of that vision from @SynBioGaoLab @sahakris @LaurieBoyer1221 @BashorLab @yogeshgoyallab @genegirl007 @isaacbhilton @Pulin_Li @jasonhyang @echaikof and many more! Link below:
Katie Galloway
@GallowayLabMITOct 21
What if a single RNA transfection could program human stem cell fate or enable functional biosensing for more than a month? Our new @CellRepMethods paper explores how self-amplifying RNA makes this possible, without genomic integration. 1/n :🧵
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Integrating synthetic biology to understand and engineer the heart, lung, blood, and sleep systems "By integrating genome engineering, dynamic gene circuits, and high-dimensional biosensors, these advances support scalable, quantitative models of multicellular biology, expanding the need for systems-level models and integration. We highlight emerging systems such as tunable transcriptional regulators, synthetic organizers, and feedback circuits that bridge molecular control with functional outcomes." tinyurl.com/LungHeartBloodSl…
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What if a single RNA transfection could program human stem cell fate or enable functional biosensing for more than a month? Our new @CellRepMethods paper explores how self-amplifying RNA makes this possible, without genomic integration. 1/n :🧵
Cell Reports Methods@CellRepMethodsSep 16
Self-amplifying RNA enables rapid, durable, integration-free programming of hiPSCs dlvr.it/TVWHCk

Self-amplifying RNA enables rapid, durable, integration-free programming of hiPSCs

Self-amplifying RNAs (saRNAs) have the potential for rapid, durable, non-integrating delivery of transgenes in human induced pluripotent stem cells. Della Santina et al. show that transcription...

cell.com
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Want to know more? You can read the paper here: dlvr.it/TVWHCk

Self-amplifying RNA enables rapid, durable, integration-free programming of hiPSCs

Self-amplifying RNAs (saRNAs) have the potential for rapid, durable, non-integrating delivery of transgenes in human induced pluripotent stem cells. Della Santina et al. show that transcription...

cell.com
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@threadreaderapp unroll
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Katie Galloway retweeted
Calin Plesa@calinplesaOct 19
We have two faculty positions open in synthetic biology (one experimental and one computational), both very broadly defined. Deadline is Oct 31. The Dept of Bioengineering in the Knight Campus at UOregon is a great place to work. Come join us! Link below.
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Nifty new paper on supercoiling influence in C. elegans! "Genome architecture shapes transcriptional responses to topoisomerase depletion in C. elegans." work from Morao et al. link below 👇
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Highlights •TOP-1 is the dominant topoisomerase supporting transcription in C. elegans •Gene orientation shapes transcriptional responses to topoisomerase depletion •Topoisomerase depletion preferentially impairs transcription of long genes •Topoisomerase depletion shortens poly(A) tails and alters promoter chromatin xitter.cf/CellReports/status/211…
Cell Reports@CellReportsOct 16
Genome architecture shapes transcriptional responses to topoisomerase depletion in C. elegans dlvr.​it/TVxZlg
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