Providing nextRAD genotyping services, and Oxford Nanopore microbial & plasmid sequencing with the plasmidsaurus service
Eugene, OR
Joined November 2012
- Tweets333
- Following111
- Followers372
- Likes295
SNPsaurus retweeted
For bacterial genome sequencing, would you pick fast, good, or cheap? Now you can get ALL 3 with our new ONT sequencing service that almost always produces circular, full-length bacterial chromosome contigs with consensus accuracy >Q50 -- often with their full plasmids too! (1/3)
SNPsaurus retweeted
Today seems like a good day to announce some improvements we've had in the works:
1. We promised lower prices as our sequencing volume increased, and thanks to our wonderful customers, as of right now we are dropping our price from $25 to $15/sample! (1/6)
A surprising fraction of the plasmids we sequence have an unexpected assembly size. Here is a good example:
Here is a nice example of the power of long-read full-plasmid sequencing such as the service offered by @SNPsaurus. We recently ordered a 4.7kbp plasmid from @Addgene. As part of our QC we sent it in for full-plasmid sequencing as part of a batch of plasmids... 1/n
SNPsaurus retweeted
This article hits a pet peeve of mine. It says "RADSeq" in the title but it uses the ddRAD method. This is important because the study is a diverse genus of moth. Not a diverse species, but 43 samples and 32 species. Why does that matter? 1/n
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SNPsaurus is excited to offer a new @PacBio service: full-length sequencing of individual plasmids for as low as $15/plasmid. No need to order primers to walk into a long insert. No charge if the plasmid isn't returned as a single sequence! snpsaurus.com/plasmid-sequen…
The @PacBio Sequel II just keeps getting better and our pricing just keeps getting better. PacBio bacterial genomes starting at $200/sample. Yeast $300/sample. A 1 Gbp genome for $1800. Library prep, sequencing, assembly and annotation included. snpsaurus.com/pacbio-small-g…
It is always fun to read the amazing research associated with samples sent to us for sequencing. Great paper!
Very happy to have our work published today! Huge thanks to @ajverster, @DanicaSchmidtk1, Joseph Mougous, Elhanan Borenstein and many others not on Twitter. nature.com/articles/s41586-0…
We were some of the original developers of RAD-Seq, but we think that for species with genome sizes under 600 Mb it now makes more sense to do whole genome genotyping (WGS at 10X read depth)--see some example project pricing below or at snpsaurus.com/whole-genome-g… #RADSeq
We offer HiFi library prep and sequencing...ask us for pricing on your specific project snpsaurus.com/pacbio-small-g…
According to Evan Eichler & co, there are 3 essential strengths of #HiFiReads over CLR technology: reduced compute time to generate a de novo assembly, superior assembly accuracy, & improved ability to assemble the most difficult regions of the genome. ow.ly/RKvb50vvvXJ
SNPsaurus retweeted
We had several requests for extension - so now closing this Sunday (Jul 14 PDT)!
calacademy.snaphire.com/jobd…
Looking for a postdoc (2.5yrs) to join my lab at the @calacademy! Come work with us on an exciting new #HopeForReefs project, leveraging genomics to study reef-wide patterns of scleractinian coral diversity. Please RT 🙏
Ad: calacademy.snaphire.com/jobd…
Lab: pimbongaerts.com/people 🚧
SNPsaurus retweeted
@NEBiolabs Q5 High-Fidelity DNA Polymerase is gaining ground on Phusion polymerase, according to citations in the literature. Visit labblab.com for insights like these and try our tools to identify which researchers are using different kits and products.
Very excited about upcoming access to @PacBio Sequel II long reads! We have changed our pricing for genome sequencing projects (see picture for some example projects). Contact us at [email protected] to discuss your particular project. snpsaurus.com/pacbio-small-g…
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SNPsaurus retweeted
Indeed, it often makes sense to do @PacBio microbial projects at service providers like @SNPsaurus @GenomeScience @umngenomics where the per microbe cost has come down significantly with multiplexing on the Sequel System
With the new longer reads and higher output from @PacBio we can pass the lower costs for de novo PacBio microbial sequencing along to you. Full service extraction, library prep and assembly/annotation informatics starting at $389/sample. snpsaurus.com/pacbio-small-g…
SNPsaurus retweeted
The RAD-seq paper just passed 2000 citations. Great group of people involved--Nate Baird (now at @illumina) and Paul Etter (at @SNPsaurus with me) worked through the details and Bill Cresko @wcresko gave insights and collegiality. Pleased @PLOSONE published it w/ wide readership.
Good advice about types of artifacts from RAD methods. Defining loci "clusters" is ripe for artifacts...I think getting draft or better assemblies is a good way to avoid some artifacts that result from "flying blind" with a de novo reference. xitter.gq/JonPuritz/status/10166…
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SNPsaurus is now offering Illumina PE 150 sequencing of bacterial genomes: library prep, sequencing to 60X read depth, assembly, annotation, and alignment to a reference with variant calling--starting at $69/sample. See snpsaurus.com/illumina-bacte…