SNPsaurus

Even little Billy of the Family Circus wishes he could be doing some long-read genome sequencing with the @PacBio Sequel, it appears!

Maybe someday soon! But until then:

We were some of the original developers of RAD-Seq, but we think that for species with genome sizes under 600 Mb it now makes more sense to do whole genome genotyping (WGS at 10X read depth)--see some example project pricing below or at snpsaurus.com/whole-genome-g… #RADSeq

Interested in bacterial methylation with @PacBio? Just add $25 per sample to get a boost in read depth to 100X and additional bioinformatics to find enriched motifs in the genome. Regular assembly/annotation starting at $300/sample and now methylation projects starting at $325.

We like @PacBio as a single-step for a closed genome (most of the genomes we sequence get a single, complete contig). Here's some BUSCOs of a recent run (F is fragmented, which is what you get from a frameshift error). We sometimes polish w/Illumina and see a few dozen changes.

Added HIFI accurate reads to our PacBio services. Bacteria starting at $300/sample; yeast $400, C. elegans $600. Rethink @PacBio being for the final step of projects and consider it a single-platform solution for excellent genome information in single samples or populations.

Very excited about upcoming access to @PacBio Sequel II long reads! We have changed our pricing for genome sequencing projects (see picture for some example projects). Contact us at [email protected] to discuss your particular project. snpsaurus.com/pacbio-small-g…

@wcresko Have you discovered TMBGs kiddie albums yet?youtube.com/watch?v=3JdWlSF1… and youtube.com/watch?v=sLkGSV9W…

@Dereneaton New internal sites are the main cause of locus drop in ddRAD, see pic, so ddRAD site size is 28 bp!